Ulated RSDs varied from 2.5-12 , which is adequate for the aims of this study. The system response was found to be linear within the investigated concentration ranges, as shown in Table 1,Table 1 Retention times, quantifier ions, LODs, RSDs, coefficients of variation and linear ranges of compounds under studyVOC Dimethyl sulfide Isoprene 2-Propenal, 2-methylPropanal, 2-methylSulfide, ethyl methyl 2-Ethylacrolein Butanal, 3-methylButanal, 2-methyl2-Pentanone n-Propyl acetate Thiophene, 3-methyl2-Heptene Propane, 2-methyl-1-(methylthio)n-Hexanal n-Propyl propionate n-Butyl acetate 3-Heptanone 2-Heptanone Benzaldehyde Furan, 2-methyl-5-(methylthio)3-Octanone 2-Nonanone CAS 75-18-3 78-79-5 78-85-3 78-84-2 624-89-5 922-63-4 590-86-3 96-17-3 107-87-9 109-60-4 616-44-4 14686-13-6 5008-69-5 66-25-1 106-36-5 123-86-4 106-35-4 110-43-0 100-52-7 13678-59-6 106-68-3 821-55-6 Rt [min] 16.35 18.18 19.11 19.43 20.84 23.19 23.48 23.53 24.10 24.88 26.02 25.11 27.48 27.83 28.11 28.27 30.60 30.78 30.99 31.05 33.47 36.25 56 75 56 85 43 106 128 99 58 0.2 0.03 0.04 0.03 0.03 0.05 0.03 0.1 0.07 43 43 97 44 0.14 Quantifier ion 62 67 70 72 61 LOD [ppb] 0.08 0.04 0.03 0.3 0.02 RSD [ ] 6 4.5 8 9 6 Not quantified 9 0.978 0.4-350 R2 0.997 0.999 0.993 0.977 0.999 Linear range [ppb] 0.24-70 0.12-12 0.1-12 0.9-150 0.06-5.Not quantified, RT confirmed 0.05 0.06 0.01 7 3 3 Not quantified Not quantified 9 10 10 2.5 7 12 7 7 11 0.994 0.996 0.997 0.997 0.998 0.998 0.988 0.981 0.974 0.6-15 0.1-7 0.12-8 0.09-7 0.09-6.5 0.15-12 0.09-8 0.3-5.5 0.21-5.7 0.998 0.998 0.998 0.15-9 0.18-9 0.03-9.Mochalski et al. Cancer Cell International 2013, 13:72 http://www.cancerci/content/13/1/Page 3 ofwith the coefficients of variation ranging from 0.974 to 0.999.HepG2 cellsThe total number of the HepG2 cells and their viability after 24 hours of incubation in the sealed measurement bottles is presented in Table 2. Cell numbers ranged from 2.606 to 30.706 (mean 17.906), whereas the viability varied from 80.1 to 99.6 (mean 91.1 ). The applied experimental conditions thus did not significantly affect the viability of cells and it can consequently be assumed that the released and consumed species reflect the normal metabolism of cells under study.Uptake of VOCs by HepG2 cellsThe uptake of volatiles by HepG2 cells from the culture medium was a matter of interest, as it can give valuable insights into the metabolism of the cells under study.Quinine A total of nine compounds were found to be metabolised in the HepG2 cell cultures as compared to blank samples (Wilcoxon signed-rank test, p 0.Etravirine 05).PMID:24406011 The detection and quantification incidences as well as the observed concentration ranges in media and cell cultures are given in Table 3. The majority of them were aldehydes including the following six representatives: 2-methyl 2propenal, 2-methyl propanal, 2-ethylacrolein, 3-methyl butanal, n-hexanal and benzaldehyde. Apart from these there were two esters (n-butyl acetate and n-propyl propionate) and the hydrocarbon isoprene. In the case of 2ethylacrolein the identification was based exclusively on the NIST mass spectral library match and the Wilcoxon test was performed using uncalibrated peak areas. The levels of 2-methyl butanal were also found to decrease, however, a proper integration and quantification of this compound was not possible due to the poor separation from 3-methyl butanal and the absence of unique ions that could be used for these purposes. Interestingly, saturated aldehydes were taken up more.
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