Onucleotides with different sequences can readily be synthesized on surfaces, for example, by light directed methods12 or a physical masking method9,10. Duplexes formed from dendritic DNA have unexpectedly high thermal stability; the melting temperature is substantially higher than that of linear counterparts. It is an effect of combining the binding forces of individual duplexes by tying their ends together in the dendritic structure.
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with an array of complementary oligonucleotides on a solid support, duplex stability is greater than that of unbranched molecules of equal length. Enhanced stability of DNA dendrimers makes them useful as building blocks for the `bottom up’ approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target.
References:
1. G.R. Newcome, C.N. Moorefield, and F. Vogtle, Dendritic Molecules: Concepts, Synthesis, Perspectives; VCH Publishers: 1996. 2. O. Boussif, et al., Proc Natl Acad Sci U S A, 1995, 92, 7297-301. 3. J.F.G.A Jansen, et.al., Science, 1994, 266, 1226. 4. T. Horn and M.S. Urdea, Nucleic Acids Res, 1989, 17, 6959-67. 5. M.L. Collins, et al., Nucleic Acids Res, 1997, 25, 2979-84. 6. M.S. Shchepinov and E.M. Southern, Russ. J. Bioorg. Chem., 1998, 24, 794. 7. M.S. Shchepinov, I.A. Udalova, A.J. Bridgman, and E.M. Southern, Nucleic Acids Res, 1997, 25, 4447-4454. 8. C. Lehmann, Y.Z. Xu, C. Christodoulou, Z.K. Tan, and M.J. Gait, Nucleic Acids Res., 1989, 17, 2379. 9. E.M. Southern, et al., Nucleic Acids Research, 1994, 22, 1368-1373. 10. E.M. Southern, U. Maskos, and J.58-85-5 IUPAC Name K. Elder, Genomics, 1992, 13, 1008-17. 11. M.S. Shchepinov, K.U. Mir, J.K. Elder, M.D. Frank-Kamenetskii, and E.M. Southern, Nucleic Acids Res, 1999, 27, 3035-41. 12. A.C. Pease, D. Solas, E.J. Sullivan, M.T. Cronin, C.P. Holmes, and S.P. Fodor, Proc Natl Acad Sci U S A, 1994, 91, 5022-6. 13. G.M. Whitesides, J.P. Mathias, and C.T. Seto, Science, 1991, 254, 1312-9.
Conclusions
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Nano-structures from DNA Dendrimers
Controllable formation of nanoscale architectures in solution and on solid supports is central to a range of activities in the emerging field of nanotechnology13.47931-85-1 site DNA molecules are well suited for these purposes because of their unique molecular recognition features.PMID:31038855 For example, dendrimers with arms terminating in oligonucleotides of the same or of different sequences could be used to build cages, cryptands, tubes, nets, scaffolds and other more complex 3-D structures11, shown in Scheme 4. A different range of structures is possible when dendrimers interact with oligonucleotides bound to a solid support, which essentially represent a dendrimer with an infinite number of branches and a single core. In this case, the formation of an infinite network is not possible. When all oligonucleotides on the surface are involved in duplex formation, the complements will form a monolayer on
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Plain and mixed oligonucleotide dendrimers can be synthesized using novel doubling and trebling phosphoramidite synthons. Incorporation of label using -32PATP and polynucleotide kinase increases in proportion to the number of 5′-ends. Fluorescent signal also increases in proportion to the number of 5′-ends, if spacers are incorporated between the labels and the ends of the branches. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it ea.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com