P. Heffernan, A. TRAIL/TNFSF10 Protein supplier Protopopov, E. Ivanova, J. E. Mahoney, M. Kost-AlimovaP. Heffernan,

P. Heffernan, A. TRAIL/TNFSF10 Protein supplier Protopopov, E. Ivanova, J. E. Mahoney, M. Kost-Alimova
P. Heffernan, A. Protopopov, E. Ivanova, J. E. Mahoney, M. Kost-Alimova, S. R. Perry, R. Bronson, R. Liao, R. Mulligan, O. S. Shirihai, L. Chin, R. A. DePinho, Telomere dysfunction induces metabolic and mitochondrial compromise. Nature 470, 359sirtuininhibitor65 (2011). E. Sahin, R. A. DePinho, Axis of ageing: Telomeres, p53 and mitochondria. Nat. Rev. Mol. Cell Biol. 13, 397sirtuininhibitor04 (2012). A. Safdar, J. M. Bourgeois, D. I. Ogborn, J. P. Small, B. P. Hettinga, M. Akhtar, J. E. Thompson, S. Melov, N. J. Mocellin, G. C. Kujoth, T. A. Prolla, M. A. Tarnopolsky, Endurance exercise rescues progeroid aging and induces systemic mitochondrial rejuvenation in mtDNA mutator mice. Proc. Natl. Acad. Sci. U.S.A. 108, 4135sirtuininhibitor140 (2011). E. Jean, D. Laoudj-Chenivesse, C. Notarnicola, K. Rouger, N. Serratrice, A. Bonnieu, S. Gay, F. Bacou, C. Duret, G. Carnac, Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. J. Cell. Mol. Med. 15, 119sirtuininhibitor33 (2011).14.15. 16. 17.18.SUPPLEMENTARY MATERIALSSupplementary material for this short article is readily available at advances.sciencemag.org/cgi/ GSK-3 beta Protein site content/full/2/7/e1600031/DC1 Supplementary Methods fig. S1. Evaluation of 7q subtelomere. fig. S2. Handle for TERRA-FISH in muscle biopsies. fig. S3. NRF1 is expressed in muscle biopsies. fig. S4. Time course of phenformin treatment in Huh-7 cells. fig. S5. Mutation of NRF1 binding websites in pGL3-10q construct. fig. S6. mPGC-1a overexpression in Huh-7 cells. fig. S7. Differentiated human myotubes. fig. S8. RNase handle for TERRA-FISH in myotubes. table S1. Overview of out there human subtelomeric sequences. table S2. Primers applied in this study. table S3. Antibodies made use of in this study. References (41sirtuininhibitor5) 19. 20. AND NOTES1. N. Arnoult, J. Karlseder, Complicated interactions involving the DNA-damage response and mammalian telomeres. Nat. Struct. Mol. Biol. 22, 859sirtuininhibitor66 (2015). two. E. H. Blackburn, E. S. Epel, J. Lin, Human telomere biology: A contributory and interactive issue in aging, illness dangers, and protection. Science 350, 1193sirtuininhibitor198 (2015). 3. M. Kaeberlein, P. S. Rabinovitch, G. M. Martin, Healthier aging: The ultimate preventative medicine. Science 350, 1191sirtuininhibitor193 (2015). four. C. M. Azzalin, P. Reichenbach, L. Khoriauli, E. Giulotto, J. Lingner, Telomeric repeatsirtuininhibitorcontaining RNA and RNA surveillance components at mammalian chromosome ends. Science 318, 798sirtuininhibitor01 (2007). 5. S. G. Nergadze, B. O. Farnung, H. Wischnewski, L. Khoriauli, V. Vitelli, R. Chawla, E. Giulotto, C. M. Azzalin, CpG-island promoters drive transcription of human telomeres. RNA 15, 2186sirtuininhibitor194 (2009). 6. N. Arnoult, A. Van Beneden, A. Decottignies, Telomere length regulates TERRA levels via enhanced trimethylation of telomeric H3K9 and HP1a. Nat. Struct. Mol. Biol. 19, 948sirtuininhibitor56 (2012). 7. K. Rippe, B. Luke, TERRA and also the state on the telomere. Nat. Struct. Mol. Biol. 22, 853sirtuininhibitor58 (2015). 8. A. Porro, S. Feuerhahn, P. Reichenbach, J. Lingner, Molecular dissection of telomeric repeat-containing RNA biogenesis unveils the presence of distinct and a number of regulatory pathways. Mol. Cell. Biol. 30, 4808sirtuininhibitor817 (2010). 9. R. L. Flynn, R. C. Centore, R. J. O’Sullivan, R. Rai, A. Tse, Z. Songyang, S. Chang, J. Karlseder, L. Zou, TERRA and hnRNPA1 orchestrate an RPA-to-POT1 switch on telomeric singlestrand.