with soil samples from agriculturally inside the M sterland M sterland area. ErrorCDK4 Inhibitor MedChemExpress indicate normal deviation (n = three). (B)(n =base (B) MS base peak chromatogramsupernatant of a soil slurry incubated soil bars indicate standard deviation MS 3). peak chromatogram with the extracted with the extracted supernatant of a with 1 mM cholate 1 mM cholate(prime) about 48 h (top) asion chromatograms withchromatograms using the (383 Da slurry incubated with for about 48 h for in addition to extracted nicely as extracted ion the m/z values of HOCDA m/z values for [M-H]-1, middle) and DOCDA (XX, 385 Da for [M-H]-1, bottom). Samples had been measured in negative MS mode. (C) 3D of HOCDA (383 Da for [M-H]-1 , middle) and DOCDA (XX, 385 Da for [M-H]-1 , bottom). Samples have been measured in UV chromatogram of the extracted supernatant of a soil slurry incubated with 1 mM cholate for about 48 h and structure damaging MS mode. quite a few intermediates assigned to peaks. Intensity is shown as aa soilmap. Red indicates with 1 mM cholate recommendations for (C) 3D UV chromatogram from the extracted supernatant of heat slurry incubated highest absorpfor about (D)h and structure suggestions for numerous in (B,C). Massesassigned to peaks. Intensity) is shown as a heat map. tion. 48 Candidate structures for peaks a-i found intermediates and absorption maxima (max had been determined by HPLC-MS measurements. Structure recommendations are primarily based for peaks a-i identified absorption spectra, and retention maxima Red indicates highest absorption. (D) Candidate structures on molecular masses, in (B,C). Masses and absorptiontime. 1,4 4,six (maxCandidate structures by HPLC-MS measurements. Structure to the -pathway, and on molecular masses, absorption ) were determined belonging (blue) towards the -pathway, (red) recommendations are based (black) potentially occurring in both pathways. When structures could not be assigned unambiguously, 1,four achievable structures are4,six two depicted. XV: 7,12spectra, and retention time. Candidate structures belonging (blue) for the -pathway, (red) to the -pathway, and (black) Dihydroxy-3-oxo-pregna-4-ene-carboxylate, XVI: 7-Hydroxy-3,12-dioxo-pregna-4-ene-carboxylate, XVII: 7,HIV-1 Inhibitor Molecular Weight 12-Dihydroxypotentially occurring in each pathways. When structures could XIX: be assigned unambiguously, two feasible structures are 3-oxo-pregna-4-ene-carboxylate, XVIII: 4-3,12-Diketocholate, not DOCDA (12-Hydroxy-3-oxo-pregna-4,6-diene-carboxdepicted. XV: 7,12-Dihydroxy-3-oxo-pregna-4-ene-carboxylate, XVI: 7-Hydroxy-3,12-dioxo-pregna-4-ene-carboxylate, XVII: ylate, XX: 3,12-Dioxo-4,6-choldienoate). 7,12-Dihydroxy-3-oxo-pregna-4-ene-carboxylate, XVIII: 4 -3,12-Diketocholate, XIX: DOCDA (12-Hydroxy-3-oxo-pregna-4,64. Discussion diene-carboxylate, XX: three,12-Dioxo-4,6-choldienoate). Aerobic bacterial degradation of 7-hydroxy bile salts in soil and water can proceed via two pathway variants, namely the 1,4-variant and also the 4,6-variant [6]. The four,6-variantMicroorganisms 2021, 9,15 of4. Discussion Aerobic bacterial degradation of 7-hydroxy bile salts in soil and water can proceed through two pathway variants, namely the 1,four -variant and also the four,six -variant [6]. The four,six variant is prevalent in the Sphingomonadaceae and differs from the 1,four -variant, that is located in other Proteobacteria and Actinobacteria, particularly in the degradation with the side chain [11,23], when the cleavage in the steroid skeleton was proposed to proceed by means of 9,10-seco cleavage in each variants. In Sphingobium sp. strain Chol11, DHSATD (XI) is the anticipated 9,
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