7.30; found: C, 82.78, H, 7.31 . Methyl two,three,4-tri-O-cinnamoyl-6-O-myristoyl–Dgalactopyranoside (eight). FTIR (KBr) (max): 1702 (-CO) cm-1. 1H-NMR (CDCl3, 400 MHz) ( ppm): H 7.75 7.52, 7.37 (three 1H, three d, J = 16.0 Hz, three PhCH = CHCO-), 7.54 (6H, m, Ar ), 7.28 (9H, m, Ar ), six.55, 6.16, six.07 (three 1H, 3 d, J = 16.1 Hz, 3 PhCH = CHCO-), five.48 (1H, d, J = 8.2 Hz, H-1), five.34 (1H, dd, J = 8.two and 10.6 Hz, H-2), five.05 (1H, dd, J = three.two and ten.six Hz, H-3), four.66 (1H, d, J = 3.7 Hz, H-4), four.40 (1H, dd, J = 11.2 and 6.6 Hz, H-6a), 4.01 (1H, dd, J = 11.2 and six.8 Hz, H-6b), 3.52 (1H, m, H-5), 3.50 (3H, s, 1-OCH3), two.32 2H, m, CH 3(CH 2) 11CH 2CO-, 1.63 2H, m, CH3(CH2)HDAC1 drug 10CH2CH2CO-, 1.25 20H, m, CH3(CH2)10CH2CH2CO-, 0.88 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 795.97. Anal Calcd. for C48H58O10: C, 72.52, H, 7.35; found: C, 72.53, H, 7.37 .Methyl 6-O-myristoyl-2,3,4-tri-O-(p-toluenesulfonyl)–Dgalactopyranoside (9). FTIR (KBr) (max): 1705 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz) ( ppm): H 8.03 (3 2H, m, Ar ), 7.94 (three 2H, m, Ar ), five.23 (1H, d, J = eight.two Hz, H-1), 5.08 (1H, dd, J = eight.0 and ten.five Hz, H-2), 4.77 (1H, dd, J = three.1 and ten.six Hz, H-3), four.53 (1H, d, J = three.7 Hz, H-4), four.27 (1H, dd, J = 11.0 and 6.five Hz, H-6a), 4.11 (1H, dd, J = 11.1 and 6.eight Hz, H-6b), three.98 (1H, m, H-5), 3.46 (3H, s, 1-OCH3), two.37 2H, m, CH3(CH2)11CH2CO-, 1.63 2H, m, CH3(CH2)10CH2CH2CO-, 1.27 20H, m, CH3(CH2)10CH2CH2CO-, 0.98 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 868.10. Anal Calcd. for C42H58O13S3: C, 58.17, H, six.74; discovered: C, 58.19, H, 6.76 . Methyl 2,3,4-tri-O-(3-chlorobenzoyl)-6-O-myristoyl-D-galactopyranoside (ten). FTIR (KBr) (max): 1709 cm-1 (C = O). 1H-NMR (CDCl3, 400 MHz): H eight.05 (3H, m, Ar ), 7.96 (3H, m, Ar ), 7.55 (3H, m, Ar ), 7.38 (3H, m, Ar -H), five.63 (1H, d, J = eight.1 Hz, H-1), five.21 (1H, dd, J = 8.two and ten.six Hz, H-2), 5.01 (1H, dd, J = 3.1 and ten.6 Hz, H-3), 4.65 (1H, d, J = 3.7 Hz, H-4), 4.40 (1H, dd, J = 11.1 and 6.six Hz, H-6a), 4.20 (1H, dd, J = 11.two and 6.8 Hz, H-6b), four.00 (1H, m, H-5), three.46 (3H, s, 1-OCH3), 2.35 2H, m, CH3(CH2)11CH2CO-, 1.65 2H, m, CH3(CH2)10CH2CH2CO-, 1.24 20H, m, CH3(CH2)10CH2CH2CO-, 0.86 3H, m, CH3(CH2)12CO-. LC S [M + 1]+ 821.19. Anal Calcd. for C42H49O10Cl3: C, 61.50, H, six.02; discovered: C, 61.52, H, 6.03 .Antimicrobial screeningThe fifteen modified thymidine derivatives (20) were subjected to antibacterial screening utilizing five bacterial strains: two Gram-positive strains, namely, Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538, and three Gram-negative strains, namely, Escherichia coli ATCC 8739, Salmonella abony NCTC 6017 and Pseudomonas aeruginosa ATCC 9027. Each of the compounds have been dissolved in dimethylformamide (DMSO) to obtain a two option (w/v). On top of that, antifungal activities from the compounds were studied against two fungi strains, namely, Aspergillus niger ATCC 16,404 and Aspergillus flavus ATCC 204,304. These test micro-organisms (bacteria and fungi) were obtained from the Division of Microbiology, University of Chittagong, Abl Source Bangladesh. Disks soaked in DMSO have been applied as the negative control.Screening of antibacterial activityThe antibacterial spectra on the test derivatives were obtained in vitro by the disk diffusion technique [29]. This approach employed paper disks of 4 mm diameter along with a glass Petri-plate of 90 mmGlycoconjugate Journal (2022) 39:261diameter all through the experiment. Sterile five (w/v) dimethyl sulfoxide (DMSO) answer ready the synthesized compounds’ preferred concentration and regular antibiotics. The pa
Posted inUncategorized